文献类型：期刊文献

英文题名：Identifying natural derived upregulators of human apoA-I expression via a cell-based drug screening system

作者：Deng, Ting[1];Ji, Wei[1];Lian, Ji-Hong[1];Guo, Lei[1];Hu, Wei-Rong[1];Qian, Ming[1];Gong, Bang-qiang[1]

机构：[1]E China Univ Sci & Technol, Coll Bioengn, Dept Biochem & Bioengn, Shanghai 200237, Peoples R China

年份：2008

卷号：46

期号：9

起止页码：610

外文期刊名：PHARMACEUTICAL BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000259105000006)】；

语种：英文

外文关键词：apoA-I; baicalein; drug screening; HDL; reverse cholesterol transport

摘要：Apolipoprotein A-I (apoA-I), the major protein component of high-density lipoprotein, is instrumental in promoting efflux of cholesterol from extrahepatic tissues to the liver. It has been proposed that upregulation of apoA-I gene expression could have anti-atherosclerosis effects. For the purpose of screening human apoA-I expression upregulators, we established a cell-based drug screening model in vitro to identifying the effects of natural derived upregulators of apoA-I. In the work, human apoA-I promoter gene was cloned into pGL3B-neo vector containing luciferase gene and neomycin resistance gene. The recombinant reporter gene vector pGL3B-neo/apo was transfected into HepG2 cells, and therefore the stable cell line SApoA-I was obtained. These cells were treated with extracts from traditional Chinese herbs. The luciferase activities were detected to identify which ones can activate apoA-I promoter. Baicalein extracted from dried roots of Scutellaria baicalensis Georgi (Labiatae, Scutellaria) showed significant dose-dependent upregulatory activity in cell model, which might become an effective basic compound for developing novel HDL-raising drugs because of its effects on proteins involved in reverse cholesterol transport.