文献类型：期刊文献

英文题名：A lithium-ion-active aerolysin nanopore for effectively trapping long single-stranded DNA

作者：Hu, Zheng-Li[1];Li, Meng-Yin[1];Liu, Shao-Chuang[1];Ying, Yi-Lun[1];Long, Yi-Tao[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2019

卷号：10

期号：2

起止页码：354

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20190206355929);WOS:【SCI-EXPANDED(收录号:WOS:000454938900034)】；

基金：This research was supported by the "Chen Guang" Project from the Shanghai Municipal Education Commission and Shanghai Education Development Foundation (17CG27), National Natural Science Foundation of China (21421004 and 61871183) and the Fundamental Research Funds for the Central Universities (222201718001, 222201717003, 222201714012).

语种：英文

外文关键词：Molecular dynamics - Oligonucleotides - Potassium compounds - Lithium Chloride - Nanopores

摘要：Wild-type aerolysin (AeL) nanopores allow direct single nucleotide discrimination of very short oligonucleotides (<= 10 nt) without labelling, which shows great potential for DNA sensing. To achieve real applications, one major obstacle of AeL is its poor capture ability of long single-stranded DNA (ssDNA, >10 nt). Here, we have proposed a novel and robust strategy for the electrostatic focusing of long ssDNA into a lithium-chloride (LiCl)-active AeL. By using this method, for the first time we have demonstrated AeL detection of ssDNA longer than 100 nt. Due to screening more negative charges, LiCl improves AeL capture ability of long ssDNA (i.e. 60 nt) by 2.63- to 10.23-fold compared to KCl. Further calculations and molecular dynamics simulations revealed that strong binding between Li+ and the negatively charged residue neutralized the AeL, leading to a reduction in the energy barrier for ssDNA capture. These findings facilitate the future high-throughput applications of AeL in genetic and epigenetic diagnostics.