文献类型：期刊文献

英文题名：Characterization of the inflammasome component SmASC in turbot (Scophthalmus maximus)

作者：Wang, Wenhui[1];Tan, Jinchao[1];Wang, Zhuang[1];Zhang, Yuanxing[1,2];Liu, Qin[1,2];Yang, Dahai[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2020

卷号：100

起止页码：324

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000523603600035)】；

基金：This work was supported by the National Key Research and Development Program of China No. 2018YFD0900500 and the Fundamental Research Funds for the Central Universities No. 222201714022 (D.Y.). Dahai Yang was supported by the Young Elite Scientists Sponsorship Program by CAST No. 2016QNRC001, Shanghai Chenguang Program No.16CG33, and the Talent Program of East China University of Science and Technology, Shanghai, China.

语种：英文

外文关键词：Scophthalmus maximus; SmASC; Edwardsiella piscicida; Immune responses

摘要：Apoptosis-associated speck-like protein containing a C-terminal caspase recruit domain (ASC) is an important adapter protein in the inflammasome complex that mediates inflammatory caspase activation and host innate immunity in mammals. However, the function of inflammasome components in lower vertebrate remains poorly understood. In this study, full length of SmASC was cloned from turbot (Scophthalmus maximus). Through bioinformatic analysis, we found that SmASC shares relatively high identity with ASC in bony fish. Furthermore, we found that the intact SmASC can form an oligomeric speck-like structure, while the PYD segment of SmASC can form the filamentous structure. Moreover, expression of SmASC was induced after intraperitoneal injection of Edwardsiella piscicida (E. piscicida) in vivo. To further explore the role of SmASC during infection, we constructed SmASC knockdown and overexpression models by administration of siRNA and overexpression plasmids in vivo, respectively. Expression of SmASC decreased the propagation of E. piscicida in different immune organs. In summary, our results characterize the function of SmASC in S. maximus, suggesting that the SmASC plays a critical role in turbot immune responses.