文献类型：期刊文献

英文题名：Lysosomal polarity increases with aging as revealed by a lysosome- targetable near -infrared fluorescent probe

作者：Shi, Donglei[1];Hu, Linghao[1];Li, Xinming[1];Liu, Wenwen[1];Gao, Ying[2];Li, Xiaokang[1];Jiang, Bei[3];Xia, Conglong[3];Guo, Yuan[2];Li, Jian[1,3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Northwest Univ, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710127, Peoples R China;[3]Dali Univ, Coll Pharm & Chem, Inst Mat Med, 5 Xue Ren Rd, Dali 671000, Peoples R China

年份：2020

卷号：319

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20202108701280);WOS:【SCI-EXPANDED(收录号:WOS:000539982800007)】；

基金：This work was supported by the National Natural Science Foundation of China (81872747, 21672064, 21977082), the National Key R&D Program of China (2017YFB0202600), the Innovative Research Team of High-level Local Universities in Shanghai, the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), the Natural Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China (No. 2020JC-38), Open Funding Project of the State Key Laboratory of Bioreactor Engineering (2018OPEN12), Technology Plan Project of Xi'an (GXYD18.1) and the Shanghai Sailing Program (17YF1403600).

语种：英文

外文关键词：Fluorescence spectroscopy - Cytology - Infrared devices - Probes - Fluorescence

摘要：Cellular senescence, the major inducer of aging contributing to chronic diseases and age-related dysfunctions, is characterized by remarkable variations in the morphology and functionality of cellular organelles. Increases in lysosomal number, pH and size constitute the most characteristic variations observed with senescence, in which increased pH can further impact the microenvironment of lysosomes. These senescence-related alterations in lysosomes causally induce changes in lysosomal polarity. However, the relationship between lysosomal polarity and senescence remains a "grey area". To this end, we presented a series of curcumin-based polarity fluorescent probes, among which KSLP1 is a near-infrared lysosome-targeting probe that can monitor lysosomal polarity with high sensitivity without interference from other micro-environmental factors. Utilizing KSLP1, we investigated variations in polarity in drug-induced senescent cells and senescent MRC5 cells, and we discovered, for the first time, that lysosomal polarity increased in senescent cells and proposed a mechanism for variations in lysosomal polarity. Moreover, the polarity of C. elegans was monitored from days 2 to 10 of adulthood, and the results indicated that the intrinsic polarity in C. elegans increased with aging. These results demonstrated that lysosomal polarity increases with senescence, providing insight into the relationship between lysosomal polarity and the aging process. ? 2020 Elsevier B.V.