文献类型：期刊文献

英文题名：Overexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-D-gluconic acid by Gluconobacter oxydans

作者：Li, Kefei[1];Mao, Xinlei[1];Liu, Liu[1];Lin, Jinping[1];Sun, Ming[1];Wei, Dongzhi[1];Yang, Shengli[1]

机构：[1]East China Univ Sci & Technol, New World Biotechnol Inst, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：15

外文期刊名：MICROBIAL CELL FACTORIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000400056200001)】；

基金：This study was financially supported by the National Key Basic Research Development Program of China (No. 2012CB721003), the National High Technology Research and Development Program of China (No. 2012AA022201C), the Shanghai Natural Science Foundation (No. 15ZR1408600), the Natural Science Foundation of China (No. 21276084), the National Major Science and Technology Projects of China (No. 2012ZX09304009) and the Natural Science Foundation of Shanghai (No.2013ZR1412100).

语种：英文

外文关键词：2-keto-D-gluconic acid; Gluconobacter oxydans; Overexpression; Resting cell

摘要：Background: 2-keto-D-gluconic acid (2KGA) is widely used as a chemical intermediate in the cosmetic, pharmaceutical and environmental industries. Several microbial fermentation processes have been developed for production of 2KGA but these suffer from substrate/product inhibition, byproduct formation and low productivity. In previous work, we showed that 2KGA can be specifically produced from glucose (Glu) or gluconic acid (GA) by resting wildtype Gluconobacter oxydans DSM2003 cells, although substrate concentration was relatively low. In this study, we attempted to improve 2KGA productivity by G. oxydans DSM2003 by overexpressing the ga2dh gene, which encodes the membrane-bound gluconate-2-dehydrogenase enzyme (GA2DH). Results: The ga2dh gene was overexpressed in G. oxydans DSM2003 under the control of three promoters, PtufB, P-ga2dh or P-ghp0169, respectively. Among the recombinant strains obtained, G. oxydans_tufB_ga2dh showed a similar growth rate to that of the control strain and displayed the highest specific productivity of 2KGA from GA, which was increased nearly twofold compared with that of the control strain during batch biotransformation. When biocatalysis conditions were optimized, with provision of sufficient oxygen during biotransformation, up to 480 g/L GA was completely utilized over 45 h by resting cells of G. oxydans_tufB_ga2dh and 453.3 g/L 2KGA was produced. A productivity of 10.07 g/L/h and a yield of 95.3 % were obtained. Overexpression of the ga2dh gene also significantly improved the conversion of Glu to 2KGA. Under optimized conditions, 270 g/L Glu was converted to 321 g/L 2KGA over 18 h, with a yield of 99.1 % and a productivity of 17.83 g/L/h. The glucose concentrations during the batch biotransformation and the 2KGA productivities achieved in this study were relatively high compared with the results of previous studies. Conclusions: This study developed an efficient bacterial strain (G. oxydans_tufB_ga2dh) for the production of 2KGA by overexpressing the ga2dh gene in G. oxydans. Supply of sufficient oxygen enhanced the positive effect of gene overexpression on 2KGA production. Gluconobacter oxydans_tufB_ga2dh is thus a competitive species for use in 2KGA production.