文献类型：期刊文献

英文题名：Reshaping the active pocket of promiscuous lactonases for degrading bulky organophosphate flame retardants

作者：Zhang, Yuanyuan[1];Zhang, Xiaoyan[1];Cheng, Huan[1];Nina, Mario Roque Huanca[1];Ge, Jun[2];Bai, Yunpeng[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Tsinghua Univ, Dept Chem Engn, Key Lab Ind Biocatalysis, Minist Educ, Beijing 100084, Peoples R China

年份：2021

卷号：57

期号：53

起止页码：6475

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20212710592615);WOS:【SCI-EXPANDED(收录号:WOS:000659205300001)】；

基金：This work was financially sponsored by the National Key Research and Development Program of China (2016YFA0204300), National Natural Science Foundation of China (No. 22078096), and Natural Science Foundation of Shanghai (18ZR1409900).

语种：英文

外文关键词：Flame retardants

摘要：Engineering of a promiscuous lactonase via semi-rational evolution gave a 1007-fold improvement in its catalytic activity in the degradation of triphenyl phosphate (TPHP). TPHP is a typical bulky organophosphate flame retardant (OPFR) and is widely used in industry. To the best of our knowledge, this is the first artificial enzyme capable of degrading OPFRs.