文献类型：期刊文献

英文题名：Accurate Data Process for Nanopore Analysis

作者：Gu, Zhen[1,2,3];Ying, Yi-Lun[1,2];Cao, Chan[1,2];He, Pingang[3];Long, Yi-Tao[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[3]E China Normal Univ, Dept Chem, Shanghai 200241, Peoples R China

年份：2015

卷号：87

期号：2

起止页码：907

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20150700514998);WOS:【SCI-EXPANDED(收录号:WOS:000348332300013)】；

基金：We acknowledge funding from the National Natural Science Foundation of China (Grants 21327807 and 21421004). Y.-T.L. is grateful for funds from the National Science Fund for Distinguished Young Scholars of China (Grant 21125522), Shanghai Science and Technology Committee (Grant 12JC1403500), and Eastern Scholar at Shanghai Institutions of Higher Learning.

语种：英文

摘要：Data analysis for nanopore experiments remains a fundamental and technological challenge because of the large data volume, the presence of unavoidable noise, and the filtering effect. Here, we present an accurate and robust data process that recognizes the current blockades and enables evaluation of the dwell time and current amplitude through a novel second-order-differential-based calibration method and an integration method, respectively. We applied the developed data process to analyze both generated blockages and experimental data. Compared to the results obtained using the conventional method, those obtained using the new method provided a significant increase in the accuracy of nanopore measurements.