文献类型：期刊文献

英文题名：AraBAD Based Toolkit for Gene Expression and Metabolic Robustness Improvement in Synechococcus elongatus

作者：Cao, Yi-Qi[1];Li, Qian[1];Xia, Peng-Fei[1];Wei, Liu-Jing[2];Guo, Ning[1];Li, Jian-Wei[1];Wang, Shu-Guang[1]

机构：[1]Shandong Univ, Sch Environm Sci & Engn, 27 Shanda Nanlu, Jinan 250100, Shandong, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：7

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000418644100018)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21476130 and No. 21676161).

语种：英文

摘要：As a novel chemical production platform, controllable and inducible modules in Synechococcus elongatus plus the ability of working in diurnal conditions are necessary. To the endeavors, inducible promoters, such as P-Trc, have been refined from Escherichia coli, but the inducer isopropyl-beta-D-thiogalactoside may cause several side-effects. Meanwhile, to promote the efficiency, photomixotrophic cultivation has been applied in S. elongatus with the additional organic carbon sources. In this study, we developed L-arabinose based modules consisted of both the P-BAD inducible promoter and the metabolism of L-arabinose in S. elongatus, since L-arabinose is an ideal heterologous feedstock for its availability and economic and environmental benefits. As expected, we achieved homogeneous and linear expression of the exogenous reporter through the P-BAD promoter, and the biomass increased in diurnal light condition via introducing L-arabinose metabolism pathway. Moreover, the combined AraBAD based toolkit containing both the P-BAD inducible module and the L-arabinose metabolism module could obtain gene expression and metabolic robustness improvement in S. elongatus. With the only additive L-arabinose, the novel strategy may generate a win-win scenario for both regulation and metabolism for autotrophic bio-production platforms.