文献类型：期刊文献

英文题名：Dominant and Active Methanogens in the Production Waters From a High-Temperature Petroleum Reservoir by DNA- and RNA-Based Analysis

作者：Zhou, Lei[1,2];Hu, Qian-Qian[1,2];Lu, Yu-Wei[1,2];Mbadinga, Serge Maurice[1,2];Liu, Yi-Fan[1,2];Li, Xiao-Xiao[1,2];Wang, Biao[3,4];Lv, Hongmei[3,4];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Gu, Ji-Dong[5];Mu, Bo-Zhong[1,2,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[3]SINOPEC, Jiangsu Oilfield Co, Petr Engn Technol Res Inst, Yangzhou, Jiangsu, Peoples R China;[4]Res Ctr Oil & Gas Microbial Engn Jiangsu, Yangzhou, Jiangsu, Peoples R China;[5]Guangdong Technion Israel Inst Technol, Environm Engn, Shantou, Guangdong, Peoples R China;[6]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, Shanghai, Peoples R China

年份：2021

卷号：38

期号：3

起止页码：191

外文期刊名：GEOMICROBIOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000579076000001)】；

基金：This work was supported by the National Natural Science Foundation of China [No. 41403066], and the Fundamental Research Funds for the Central Universities of China [No. 222201414029].

语种：英文

外文关键词：Methanogen; petroleum reservoir; mcrA; RNA; methanogenesis

摘要：Methane metabolism in deep subsurface petroleum reservoirs is of paramount interest in the global biogeochemical cycle of carbon. Methanogenesis in such habitats is driven mostly by methanogens in syntrophic association with bacteria. In the present study, methanogenic communities in production water samples from six wells of a high-temperature petroleum reservoir were analyzed based on both genomic 16S rDNA and metabolically active 16S rRNA. The PCR-amplifiedmcrAgene analysis showed thatMethanosaeta(26%) andMethanomassiliicoccus(56%) were separately the dominant members in sample W2_71 and W9_18 at the DNA level. In comparison, the RNA-based analysis showed thatMethanosaeta(63 similar to 83%) followed byMethanolinea(16 similar to 23%) were the most active methanogens, which were different from the communities of genomic DNA. While several lines of studies indicated that CO2-reducing methanogens of the genusMethanothermobacterwas the most frequently detected phylotype in deep-subsurface petroleum reservoirs. Redundancy analysis (RDA) showed the possible correlation between active methanogens (Methanosaeta) and environmental factors (CO32-). The datasets indicated the importance of investigating methanogenic community by integrating DNA- and RNA-based approaches. These results provide new insight into active methanogens in high-temperature petroleum reservoirs, and promote a comprehensive understanding of methanogens as well as their potential methanogenic pathways in such environments.