文献类型：期刊文献

英文题名：Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe

作者：Gu, Kaizhi[1,2];Xu, Yisheng[3];Li, Hui[5];Guo, Zhiqian[1,2];Zhu, Shaojia[4];Zhu, Shiqin[1,2];Shi, Ping[4];James, Tony D.[6];Tian, He[1,2];Zhu, Wei-Hong[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[5]Shanghai Jiao Tong Univ, Dept Radiol, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China;[6]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

年份：2016

卷号：138

期号：16

起止页码：5334

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20162002392183);WOS:【SCI-EXPANDED(收录号:WOS:000375244700030)】；

基金：This work was supported by National 973 Program (2013CB733700), NSFC for Creative Research Groups (21421004) and Distinguished Young Scholars (21325625), NSFC/China, Oriental Scholarship, Scientific Committee of Shanghai (14ZR1409700 and 15XD1501400), Shanghai Pujiang Program (13PJD010), Fok Ying Tong Education Foundation (142014), the Fundamental Research Funds for the Central Universities (WK1013002 and 222201313010), Programme of Introducing Talents of Discipline to Universities (B16017), and the Catalysis and Sensing for Environment (CASE) network for research exchange opportunities. T.D.J. thanks ECUST for a guest professorship.

语种：英文

外文关键词：Enzyme activity - Probes - Infrared devices - Diseases - Fluorescence - Molecular biology - Physiological models - Physiology - Tumors

摘要：Development of "smart" noninvasive bioimaging probes for trapping specific enzyme activities is highly desirable for cancer therapy in vivo. Given that beta-galactoSidase (beta-gal) is an important biomarker for cell senescence and primary ovarian cancers, we design an enzyme-activatable ratiometric near-infrared (NIR) probe (DCM-beta gal) for the real-time fluorescent quantification and trapping of beta-gal activity in vivo and in situ. DCM-beta gal manifests significantly ratiometric and turn-on NIR fluorescent signals simultaneously in response to beta-gal concentration, which makes it favorable for monitoring dynamic beta-gal activity in vivo with self-calibration in fluorescent mode. We exemplify DCM-beta gal for the ratiometric tracking of endogenously overexpressed beta-gal distribution in living 293T cells via the lacZ gene transfection method and OVCAR-3 cells, and further realize real-time in vivo bioimaging of beta-gal activity in colorectal tumor-bearing nude mice. Advantages of our system include light-up ratiometric NIR fluorescence with large Stokes shift, high photostability, and pH independency under the physiological range, allowing for the in vivo real-time evaluation of beta-gal activity at the tumor site with high-resolution three dimensional bioimaging for the first time. Our work provides a potential tool for in vivo real-time tracking enzyme activity hi preclinical applications.