文献类型：期刊文献

英文题名：Dual-site recognition leads to a ratiometric fluorescent probe for norepinephrine: quantitative detection and visualization for in-depth understanding of hypertension

作者：Wang, Fengzhi[1];Wang, Jiawen[1];Zhu, Weiping[1];Yang, Yangyang[1];Li, Honglin[1,4];James, Tony D.[2,3];Xu, Yufang[1];Qian, Xuhong[1,4]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Univ Bath, Dept Chem, Bath BA2 7AY, England;[3]Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China;[4]East China Normal Univ, Innovat Ctr Artificial Intelligence & Drug Discove, Shanghai 200241, Peoples R China

年份：2025

卷号：16

期号：41

起止页码：19215

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20254219331170);WOS:【SCI-EXPANDED(收录号:WOS:001573289100001)】；

基金：This work is financially supported by National Key Research and Development Programs (2022YFD1700403 and 2023YFD1700303). The authors wish to thank Prof. Amilra Prasanna de Silva, for his valuable and thoughtful suggestions on this paper. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support.

语种：英文

外文关键词：Fluorescence - Neurophysiology - Tissue - Tissue engineering - Visualization

摘要：Hypertension is closely associated with the activation of sympathetic nervous system (SNS). Norepinephrine (NE) belongs to the catecholamine family and is the primary neurotransmitter of SNS. Plasma and tissue NE levels serve as biomarkers of sympathetic overdrive in hypertension, providing measurable indicators to guide therapeutic approaches. However, there are only a few reliable tools to simultaneously quantify plasma NE and visualize tissue NE for comprehensive SNS assessment in hypertension. Herein, to selectively recognize the unique l-hydroxyethylamine and catechol moieties of NE, we designed and synthesized a dual-site fluorescent probe that can specifically bind to these two characteristic groups. The recognition process generates a macrocyclic-ring enhanced by silver bridging, accompanied by a ratiometric fluorescence change. The probe enables quantitative NE detection in both plasma and urine, as well as NE visualization in heart, kidney and adrenal gland tissues. Notably, our results demonstrated that spontaneously hypertensive rats (SHR) have higher circulating and tissue NE levels, indicating SNS overactivation. These findings provide a powerful molecular tool for the assessment of SNS activity and offer an in-depth understanding of hypertension.