文献类型：期刊文献

英文题名：Bright and stable dye-doped fluorescent polystyrene microspheres: quantitative lateral flow immunoassay for detecting serum amyloid A

作者：Li, Dan[1,2];Li, Qiang[4];Ma, Delong[1,2];Yan, Chenxu[1,2];Luo, Xinyu[1,2];Luo, Qianfu[1,2];Guo, Zhiqian[1,2,3]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem,Feringa, Inst Fine Chem,Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr,Fering, Sch Chem & Mol Engn,Frontiers Sci Ctr Materiobiol, Inst Fine Chem,Joint Int Res Lab Precis Chem & Mol, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Frontier Sci Res Base Optogenet Tech Cell, Shanghai 200237, Peoples R China;[4]Natl Hlth Commiss, Natl Key Lab Intelligent Tracking & Forecasting In, Natl Inst Parasit Dis,Chinese Ctr Dis Control & Pr, Chinese Ctr Trop Dis Res,Natl Res Ctr Trop Dis, Shanghai, Peoples R China

年份：2025

卷号：13

期号：34

起止页码：10536

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20253519072023);WOS:【SCI-EXPANDED(收录号:WOS:001545181500001)】；

基金：This work was supported by NSFC, China (32394001, 22225805, 32121005, and 22378122); Shanghai Science and Technology Innovation Action Plan (No. 23J21901600); Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28); Shanghai Pujiang Program (22PJ1411800); and Science and Technology Commission of Shanghai Municipality (24DX1400200).

语种：英文

外文关键词：Body fluids - Chemical stability - Fluorophores - Immunology - Microspheres

摘要：Difluoroboron is a fundamental molecular building block that enables the development of numerous highly bright fluorophores for biosensing and imaging. However, its poor stability and limited brightness in aqueous solutions are long-standing and unresolved issues in developing difluoroboron-based fluorophores, which significantly limit their widespread biosensing applicability. Herein, we report a generalizable strategy for synthesising dye-doped fluorescent polystyrene microspheres as a local hydrophobic microenvironment to "protect" difluoroboron curcuminoids from water and reactive oxygen species. This strategy demonstrates a breakthrough in the chemical stability, photo-stability and brightness of difluoroboron-based fluorophores in aqueous solutions, making it suitable for point-of-care testing. The results of lateral flow immunoassay using the dye-doped fluorescent polystyrene microspheres highlight a wide linear range, low limit of detection, good intra-assay and inter-assay coefficient of variations; thus, the reported microspheres can be considered an efficient tool for serum amyloid A detection in human serum samples.