文献类型：期刊文献

英文题名：Acceleration effect of amino acid supplementation on glycerol assimilation by Escherichia coli in minimal medium

作者：Xiang, Gang[1];Li, Jiang[1];Duan, JiFu[1];Shao, Fei[1];Xu, Jiajie[1];Fu, Shuilin[1];Gong, Heng[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：35

期号：9

起止页码：1495

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20133216589143);WOS:【SCI-EXPANDED(收录号:WOS:000322577200019)】；

语种：英文

外文关键词：Amino acids; Cell growth; Escherichia coli; Glycerol; Lactose; Minimal medium

摘要：Growth of Escherichia coli BL21 in a glycerol minimal medium was accelerated following supplementation with trace amounts of amino acid (0.35 mM). Of 12 amino acids tested, Arg and Ser gave the highest response, increasing cell growth by 63 and 53 %, respectively, compared to control cells. The ability of amino acids to accelerate cell growth was "switch-like" and was achieved by promoting glycerol utilization, which may be applied to shorten the long lag-phase when glycerol is used as carbon source. Acceleration of cell growth following amino acid supplementation was also observed using lactose minimal medium.