文献类型：期刊文献

英文题名：Highly selective, naked-eye and fluorescent "off-on" probe for detection of histidine/histidine-rich proteins and its application in living cell imaging

作者：Zhang, Shenyi[1];Yang, Chunmei[1];Zhu, Weiping[1];Zeng, Bubing[1];Yang, Youjun[1];Xu, Yufang[1];Qian, Xuhong[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2012

卷号：10

期号：8

起止页码：1653

外文期刊名：ORGANIC & BIOMOLECULAR CHEMISTRY

收录：;EI(收录号：20120814802154);WOS:【SCI-EXPANDED(收录号:WOS:000300040800025)】；

基金：This work is financially supported by the National Basic Research Program of China (973 Program, 2010CB126100), the National High Technology Research and Development Program of China (863 Program 2011AA10A207), the National Natural Science Foundation of China (Grants 21076077), the China 111 Project (grant B07023), the Shanghai Leading Academic Discipline Project (B507) and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Amino acids - Colorimetry - Proteins - Fluorescence

摘要：A highly selective colorimetric and fluorescence enhanced probe S1 (M2@Cu) for histidine and histidine-rich proteins has been developed. In neutral aqueous ethanol solution, probe S1 can selectively detect histidine out of twenty DNA encoded amino acids by showing a color change from brownish red to light green, and with a fluorescence enhancement up to 99-fold at 537 nm, simultaneously.