文献类型：期刊文献

英文题名：Structural Diversity of the Lipopeptide Biosurfactant Produced by a Newly Isolated Strain, Geobacillus thermodenitrifcans ME63

作者：Li, Jia-Yi[1];Liu, Yi-Fan[1,2,3];Zhou, Lei[1,2];Gang, Hong-Ze[1,2,3,4];Liu, Jin-Feng[1,4,5];Sun, Gang-Zheng[6];Wang, Wei-Dong[6];Yang, Shi-Zhong[1,2];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr MEOR, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Shanghai 200237, Peoples R China;[5]Daqing Huali Biotechnol Co Ltd, Daqing 163511, Heilongjiang, Peoples R China;[6]Sinopec, Res Inst Petr Engn & Technol, Shengli Oilfield Co, Dongying 257088, Peoples R China

年份：2023

卷号：8

期号：24

起止页码：22150

外文期刊名：ACS OMEGA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001009798400001)】；

基金：The authors thank the Research Program of the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, for providing facilities and financial support. The authors acknowledge the financial support received from the National Key Research and Development Program of China (no. 2022YFC2105200), the National Natural Science Foundation of China (42061134011, 42173076, and 52074129), the Natural Science Foundation of Shanghai (21ZR1417400), and the Fundamental Research Funds for the Central Universities of China (JKJ01231714). The authors are also thankful to the School of Chemistry and Molecular Engineering, East China University of Science and Technology.

语种：英文

摘要：Thegenus Geobacillus is activein degradation of hydrocarbons in thermophilic and facultative environmentssince it was first reported in 1920. Here, we report a new strain, Geobacillus thermodenitrificans ME63, isolated froman oilfield with the ability of producing the biosurfactant. The composition,chemical structure, and surface activity of the biosurfactant producedby G. thermodenitrificans ME63 wereinvestigated by using a combination of the high-performance liquidchromatography, time-of-flight ion mass spectrometry, and surfacetensiometer. The biosurfactant produced by strain ME63 was identifiedas surfactin with six variants, which is one of the representativefamily of lipopeptide biosurfactants. The amino acid residue sequencein the peptide of this surfactin is N-Glu -> Leu -> Leu -> Val -> Leu -> Asp -> Leu-C. The criticalmicelle concentration (CMC) of the surfactin is 55 mg L-1, and the surface tension at CMC is 35.9 mN m(-1), which is promising in bioremediation and oil recovery industries.The surface activity and emulsification properties of biosurfactantsproduced by G. thermodenitrificans ME63showed excellent resistance to temperature changes, salinity changes,and pH changes.