文献类型：期刊文献

英文题名：Catalytic-Hairpin-Assembly-Assisted DNA Tetrahedron Nanoprobe for Intracellular MicroRNA Imaging

作者：Huang, Qing[1,2];Ma, Pei-Qiang[2];Li, Hua-Dong[2];Yin, Bin-Cheng[1,2,3];Ye, Bang-Ce[1,2,3]

机构：[1]Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Inst Engn Biol & Hlth, Hangzhou 310014, Zhejiang, Peoples R China;[2]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832000, Xinjiang, Peoples R China

年份：2020

卷号：3

期号：5

起止页码：2861

外文期刊名：ACS APPLIED BIO MATERIALS

收录：EI(收录号：20202808929127);WOS:【ESCI(收录号:WOS:000604588000032)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (21822402, 21675052, 31730004), the National Key Research and Development Project (2018YFA0900404), the Fundamental Research Funds for the Central Universities, and the Research Program of State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：microRNA imaging; living cells; DNA tetrahedron nanoprobe; catalytic hairpin assembly; fluorescence resonance energy transfer

摘要：The aberrant expression levels of microRNAs (miRNAs) are tightly linked with the initiation and development of various diseases and genetic disorders. Here, we reported a catalytic-hairpin-assembly-assisted DNA tetrahedron nanoprobe for intracellular miRNA detection. The target miRNA initiated the catalytic-hairpin-assembly reaction between the tetrahedron nanoprobes to generate large tetrahedron clusters with an enhanced fluorescence resonance energy transfer between the Cy3 and Cy5 dyes. The proposed nanoprobes were capable of visualizing the expression levels and spatial distributions of miRNA in living cells, providing a feasible approach in designing versatile nanostructures for reliable bioanalysis and clinical diagnosis.