文献类型：期刊文献

英文题名：Quantification of Periodontal Pathogens Cell Counts by Capillary Electrophoresis

作者：Li, Zhenqing[1];Chen, Shaoxiong[1];Liu, Chenchen[1];Zhang, Dawei[1];Dou, Xiaoming[2];Yamaguchi, Yoshinori[1,2,3]

机构：[1]Univ Shanghai Sci & Technol, Engn Res Ctr Opt Instrument & Syst, Minist Educ, Shanghai Key Lab Modern Opt Syst, Shanghai 200093, Peoples R China;[2]E China Univ Sci & Technol, Fac Sci, Dept Phys, Photon & Biomed Res Inst, Shanghai 200237, Peoples R China;[3]Osaka Univ, Grad Sch Engn, Dept Appl Phys, Yamadaoka Suita City, Osaka 5650871, Japan

年份：2014

卷号：1361

起止页码：286

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20143600006891);WOS:【SCI-EXPANDED(收录号:WOS:000342257200031)】；

基金：This research was supported by the National Natural Science Foundation of China (No. 21205078 and No. 61378060), Research Fund for the Doctoral Program of Higher Education of China (No. 20123120110002). We acknowledged on partly financial support by Grand-in-Aid for Scientific Research (Houga) (No. 25600049 (Y. Y.), JSPS, Japan.

语种：英文

外文关键词：Capillary electrophoresis; Polymerase chain reaction; Periodontal pathogens; Analytical methods; Fluorescence intensity

摘要：Gingivitis is a highly prevalent periodontal disease around the worldwide. Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic appliance therapy were quite different. We proposed a simple method to extract the periodontal pathogens from the periodontal pocket in this work and demonstrated a new approach to determine periodontal pathogen level based on capillary electrophoresis (CE). After polymerase chain reaction amplification of P.g (197 bp), T.d (311 bp), and T.f (641 bp), it shows that they can rapidly identified by CE within 5 min. The peak area in the eletropherogram is linearly related to the concentration of P.g, T.d, and T.f, and the correlation coefficients R-2 corresponding to them are 0.993, 0.993, and 0.956, respectively. According to this linearly relationship, the estimated concentration of P.g, T.d, and T.f in gingival crevicular fluid from one volunteer was inferred to be about 9.90 x 10(2), 1.48 x 10(3), and 9.01 x 10(2) cells/mu 1, respectively. (C) 2014 Elsevier B.V. All rights reserved.