文献类型：期刊文献

英文题名：Single molecule analysis of light-regulated RNA: spiropyran interactions

作者：Zhang, Xing;Zhang, Junji[1];Ying, Yi-Lun;Tian, He;Long, Yi-Tao

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：5

期号：7

起止页码：2642

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20142417812563);WOS:【SCI-EXPANDED(收录号:WOS:000337108200003)】；

基金：This work was supported by the National Base Research 973 Program (2013CB733700) and National Science Fund of China (21125522, 21327807). Y.-T. Long is supported by the Program for Professor of Special Appointment (Eastern Scholar). J. Zhang is supported by the 53rd Chinese Post-doctor Fund (2013M531138), Doctoral New Teacher Fund (20130074120002) and WJ1313004. Y.-L. Ying thanks the Sino-UK Higher Education Research Partnership for PhD Studies.

语种：英文

外文关键词：Photochromism - Binding energy

摘要：The photo-regulated interactions between an RNA aptamer and photochromic spiropyran were investigated at a single-molecule level via an alpha-hemolysin nanopore. Upon irradiation of alternating UV/visible light, the translocation process of the RNA aptamer could be optically tuned on account of its different binding affinity with two photoisomers, spiropyran and merocyanine. This provides a general analytic model for understanding the mechanism of a photo-regulated biomolecule conformational change at a single-molecule level.