文献类型：期刊文献

英文题名：Edwardsiella tarda flagellar protein FlgD: A protective immunogen against edwardsiellosis

作者：Zhang, Meng[1];Wu, Haizhen[1];Li, Xiaoyong[1];Yang, Minjun[1];Chen, Tao[1];Wang, Qiyao[1];Liu, Qin[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：30

期号：26

起止页码：3849

外文期刊名：VACCINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000304850000006)】；

基金：This work was supported by grants from the Ministry of Agriculture of China (No. nycytx-50), the National High Technology Research and Development Program of China (No. 2008AA092501) and the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204).

语种：英文

外文关键词：Edwardsiella tarda; Flagellar protein; FlgD; Protective antigen

摘要：Edwardsiella tarda is a gram-negative bacterium and a causative agent of edwardsiellosis, resulting to severe loss of the aquaculture industry. In this study, based on the reverse vaccinology, sixteen flagellar proteins were selected from highly pathogenic E. tarda EIB202 genome information and in silico analyzed as potential vaccine candidates. Among them, ten recombinant proteins were highly expressed in Escherichia coli and successfully purified. The immunoprotective potentials of these purified recombinant proteins were evaluated in zebrafish model. And recombinant FlgD and FliD were found to lead to a high relative percent survival (RPS, about 70%) against E. tarda EIB202. Furthermore, FlgD required in flagellum hook assembly brought about the similar immune protection in turbot. The immune responses of zebrafish and turbot to recombinant FlgD were also investigated, and the results indicated that its high protection was mainly involved in cellular mediated immune response, corresponding to the intracellular pathogenicity of E. tarda. (C) 2012 Elsevier Ltd. All rights reserved.