文献类型：期刊文献

英文题名：Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells

作者：Fan, Li[1];Zhao, Liang[1];Ye, Zhaoyang[1];Sun, Yating[1];Kou, Tianci[1];Zhou, Yan[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：32

期号：9

起止页码：1239

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20103413170224);WOS:【SCI-EXPANDED(收录号:WOS:000280892800007)】；

语种：英文

外文关键词：CHO cells; Culture temperature; Specific productivity; TNFR-Fc

摘要：Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37 degrees C. TNFR-Fc productivity, qTNFR-Fc, increased as culture temperature decreased; and the maximum qTNFR-Fc was 20 mg/(10(6) cells/ml) at 30 degrees C which was three times that at 37 degrees C. Increasing the viable cell density (VCD) to above 2.2 x 10(6) cells/ml, however, decreased the qTNFR-Fc at 30 degrees C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve qTNFR-Fc but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed.