文献类型：期刊文献

英文题名：Screening of a phage display library of exendin-4 mutants with the extracellular domain of rat GLP-1 receptor

作者：Yin, Xiaopu; Ma, Yushu; Liu, Meiyun; Gao, Weifeng; Wei, Dongzhi

机构：[1]E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2007

卷号：14

期号：8

起止页码：816

外文期刊名：PROTEIN AND PEPTIDE LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000250570300014)】；

语种：英文

外文关键词：exendin-4; GLP-1 receptor; phage display library

摘要：A phage display library of exendin-4 mutants was screened with the extracellular domain of rat glucagon-like peptide 1 receptor as the target. A novel variant of exendin-4 with higher affinity for the receptor fraction than that of the wild type was identified. The increased affinity was attributed to the substitution of Glu 16 by Val 16. Although the substitution probably caused an increased entropic cost to the helix region, the linker around Val 16 is more flexible resulting in the increase of affinity for the receptor.