文献类型：期刊文献

英文题名：Tailoring the P450 Monooxygenase Gene for FR-008/Candicidin Biosynthesis

作者：Chen, Shi[1,2];Mao, Xiangzhao[3];Shen, Yaling[3];Zhou, Yongjun[1,2];Li, Jialiang[1,2];Wang, Lianrong[1,2];Tao, Xinyi[3];Yang, Liang[3];Wang, Yuxiao[3];Zhou, Xiufen[1,2];Deng, Zixin[1,2];Wei, Dongzhi[3]

机构：[1]Shanghai Jiao Tong Univ, Lab Microbial Metab, Shanghai 200030, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：75

期号：6

起止页码：1778

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;EI(收录号：20091211967556);WOS:【SCI-EXPANDED(收录号:WOS:000263920900042)】；

基金：We thank Qianjin Kang for nuclear magnetic resonance data analysis and many valuable comments and Jennifer Jocz for her critical reading of our manuscript.We thank the National Science Foundation of China, the Ministry of Science and Technology (973 and 863 programs), and the Science and Technology Committee of Shanghai Municipality (grant 07DZ19503-4) for research support.

语种：英文

外文关键词：Biochemistry

摘要：Tailoring steps are often important for the activity of mature antibiotics. Here, we report that novel decarboxylated FR-008/candicidin derivatives were obtained from the P450 monooxygenase gene fscP mutant of Streptomyces sp. strain FR-008. The toxicity of decarboxylated FR-008/candicidin derivatives has been shown to be greatly reduced compared to that of wild-type FR-008/candicidin.