文献类型：期刊文献

英文题名：An injectable gel based on photo-cross-linkable hyaluronic acid and mesoporous bioactive glass nanoparticles for periodontitis treatment

作者：Hu, Zhihuan[1];Lv, Xiaolei[2];Zhang, Huan[1];Zhuang, Shiya[1];Zheng, Kai[3];Zhou, Tian[4];Cen, Lian[1]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, Dept Prod Engn, Shanghai Key Lab Multiphase Mat Chem Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Natl Clin Res Ctr Stomatol, Sch Med,Dept Oral & Maxillofacial Implantol,Shangh, Shanghai, Peoples R China;[3]Nanjing Med Univ, Jiangsu Prov Engn Res Ctr Stomatol Translat Med, Nanjing 210029, Peoples R China;[4]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Ctr Head & Neck Oncol Clin & Translat Sci, Sch Med,Dept Oral & Maxillofacial Head & Neck Onco, Shanghai, Peoples R China

年份：2024

卷号：257

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20235015219354);WOS:【SCI-EXPANDED(收录号:WOS:001137621400001)】；

基金：This study is financially supported by the Shanghai Pujiang Program (21PJD035) .

语种：英文

外文关键词：Guided bone regeneration; Photo-crosslink; Bioactive glass; Injectable gel; Periodontitis

摘要：Guided bone regeneration (GBR) is an effective strategy to promote periodontal tissue repair. The current study aimed to develop an injectable gel for GBR, composed of photo-cross-linkable hyaluronic acid and mesoporous bioactive glass nanoparticles (MBGNs) loaded with antibacterial minocycline hydrochloride (MNCl). Hyaluronic acid modified with methacrylic anhydride (MHA) that could be cross-linked under UV irradiation was first synthesized. Dynamic rheological evaluation of MHA under UV was carried out to determine its in-situ gelling feasibility and stability. Morphological and mechanical characterization was performed to determine the optimal concentration of MHA gels. Sol-gel derived MBGNs loaded with MNCl were further incorporated into MHA gels to obtain the injectable drug-loaded MBGN-MNCl/MHA gels. In vitro antibacterial, anti-inflammatory and osteogenic effects of this gel were evaluated. It was shown that the MHA gel obtained from 3 % MHA under UV treatment of 30s exhibited a suitable porous structure with a compressive strength of 100 kPa. MBGNs with particle size of -120 nm and mesopores were confirmed by TEM and SEM. MBGNs had a loading capacity of -120 mg/g for MNCl, exhibiting a sustained release behavior. The MBGN-MNCl/MHA gel was shown to effectively inhibit the proliferation of Streptococcus mutans and the expression of pro-inflammatory factors IL-6 and TNF-alpha by macrophages. It could on the other hand significantly promote the expression of osteogenicrelated genes ALP, Runx2, OPN, and osterix of MC3T3-E1 cells. In conclusion, the current design using photocrosslinkable MHA gel embedded with MNCl loaded MBGNs can serve as a promising injectable formulation for GBR treatment of irregular periodontal defects.