文献类型：期刊文献

英文题名：Overview of Regulatory Strategies and Molecular Elements in Metabolic Engineering of Bacteria

作者：Wang, Tianwen[2];Ma, Xingyuan[1];Du, Guocheng[2];Chen, Jian[2]

机构：[1]E China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 214122, Peoples R China;[2]Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

年份：2012

卷号：52

期号：3

起止页码：300

外文期刊名：MOLECULAR BIOTECHNOLOGY

收录：;EI(收录号：20130716012475);WOS:【SCI-EXPANDED(收录号:WOS:000310205100010)】；

基金：The research was financially supported by grants from the National Science Foundation of China (No. 20625619, 20836003, 31000054); the Key Project of Chinese National Programs for Fundamental Research and Development (973 program, No. 2007CB71403, 2010CB535014); the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0532); the Fundamental Research Funds for the Central Universities (No. JUSRP10917).

语种：英文

外文关键词：Metabolic pathway; Metabolic engineering; Riboswitch; Intergenic region; Rational design; Directed evolution

摘要：From a viewpoint of biotechnology, metabolic engineering mainly aims to change the natural status of a pathway in a microorganism towards the overproduction of certain bioproducts. The biochemical nature of a pathway implies us that changed pathway is often the collective results of altered behavior of the metabolic enzymes encoded by corresponding genes. By finely modulating the expression of these genes or the properties of the enzyme, we can gain efficient control on the pathway. In this article, we reviewed the typical methods that have been applied to regulate the expression of genes in metabolic engineering. These methods are grouped according to the operation targets in a typical gene. The transcription of a gene is controlled by an indispensable promoter. By utilizing promoters with different strengths, expected levels of expression can be easily achieved, and screening a promoter library may find suitable mutant promoters that can provide tunable expression of a gene. Auto-responsive promoter (quorum sensing (QS)-based or oxygen-inducible) simplifies the induction process by driving the expression of a gene in an automated manner. Light responsive promoter enables reversible and noninvasive control on gene activity, providing a promising method in controlling gene expression with time and space resolution in metabolic engineering involving complicated genetic circuits. Through directed evolution and/or rational design, the encoding sequences of a gene can be altered, leading to the possibly most profound changes in properties of a metabolic enzyme. Introducing an engineered riboswitch in mRNA can make it a regulatory molecule at the same time; ribosomal binding site is commonly engineered to be more attractive for a ribosome through design. Terminator of a gene will affect the stability of an mRNA, and intergenic region will influence the expression of many related genes. Improving the performance of these elements are generally the main activities in metabolic engineering.