文献类型：期刊文献

英文题名：Structural characterization of the polysaccharide moiety of an aqueous glycopeptide from mannatide

作者：Li, Si[1];Pan, Chen[1];Xia, Wei[1];Zhang, Wenqing[1];Wu, Shengyu[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：67

起止页码：351

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20235015204879);WOS:【SCI-EXPANDED(收录号:WOS:000336881200048)】；

基金：This project was supported by Chengdu Leer Pharmaceutical Co., Ltd. of Chengdu, China.

语种：英文

外文关键词：Mannatide; Polysaccharide; Structure

摘要：A homogeneous glycopeptide with a molecular weight of 1.61 x 10(5) Da, termed as MT2-A, was isolated from crude mannatide by DEAE-52 cellulose column and Sephacryl S-300 gel column. The polysaccharide moiety of MT2-A was mainly composed of mannose and trace amount of glucose. Besides, MT2-A contained 16 kinds of amino acids and the total amino acid content was 4.41%. The detailed chemical structure of MT2-A was elucidated using methylation analysis, Fourier transform infrared spectroscopy (FT-IR), partial acid hydrolysis, selective acetolysis and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Based on the experimental results, it was concluded that the polysaccharide moiety of MT2-A had a backbone of (1 -> 6)-alpha-D-mannopyranose residues, which highly branched at O-2 position of (1 -> 2,6)-alpha-D-mannopyranose residues. The side chains were mainly composed of (1 ->)-alpha-D-mannopyranose, (1 -> 2)-alpha-D-mannopyranose and (1 -> 3)-alpha-D-mannopyranose residues. The length of the side chains could not be longer than four mannose residues. (C) 2014 Elsevier B.V. All rights reserved.