文献类型：期刊文献

英文题名：Facile Preparation of Core-Shell Magnetic Metal Organic Framework Nanoparticles for the Selective Capture of Phosphopeptides

作者：Chen, Yajing[1];Xiong, Zhichao[1];Peng, Li[1];Gan, Yangyang[1];Zhao, Yiman[1];Shen, Jie[1];Qian, Junhong[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Dept Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：7

期号：30

起止页码：16338

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20153201115591);WOS:【SCI-EXPANDED(收录号:WOS:000359279800027)】；

基金：Financial support is gratefully acknowledged from Science Foundation for Young Scientists of China (No. 21105027), support of the National Natural Science Foundation of China (No.21475044), the Key Research Project from the Ministry of Public Security (201202ZDYJ005), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044).

语种：英文

外文关键词：magnetic nanoparticles; metal-organic framework (MOF); phosphorylated peptides; mass spectrum; size-exclusion

摘要：In regard to the phosphoproteome, highly specific and efficient capture of heteroideous kinds of phosphopeptides from intricate biological sample attaches great significance to comprehensive and in-depth phosphorylated proteomics research. However, until now, it has been a challenge. In this study, a new-fashioned porous immobilized metal ion affinity chromatography (IMAC) material was designed and fabricated to promote the selectivity and detection limit for phosphopeptides by covering a metal-organic frameworks (MOFs) shell onto Fe3O4 nanoparticles, taking advantage of layer-by-layer method (the synthesized nanoparticle denoted as Fe3O4@MIL-100 (Fe)). The thick layer renders the nanoparticles with perfect hydrophilic character, super large surface area, large immobilization of the Fe3+ ions and the special porous structure. Specifically, the as-synthesized MOF-decorated magnetic nanoparticles own an ultra large surface area which is up to 168.66 m(2) g(-1) as well as two appropriate pore sizes of 1.93 and 3.91 nm with a narrow grain-size distribution and rapid separation under the magnetic circumstance. The unique features vested the synthesized nanoparticles an excellent ability for phosphopeptides enrichment with high selectivity for beta-casein (molar ratio of beta-casein/BSA, 1:500), large enrichment capacity (60 mg g(-1)), low detection limit (0.5 fmol), excellent phosphopeptides recovery (above 84.47%), fine size-exclusion of high molecular weight proteins, good reusability, and desirable batch-to-batch repeatability. Furthermore, encouraged by the experimental results, we successfully performed the as-prepared porous IMAC nanoparticle in the specific capture of phosphopeptides from the human serum (both the healthy and unhealthy) and nonfat milk, which proves itself to be a good candidate for the enrichment and detection of the low-abundant phosphopeptides from complicated biological samples.