文献类型：期刊文献

英文题名：Co-production of 3-hydroxypropionic acid and 1,3-propanediol by Klebseilla pneumoniae expressing aldH under microaerobic conditions

作者：Huang, Yanna[1]; Li, Zhimin[1]; Shimizu, Kazuyuki[2]; Ye, Qin[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Bioscience and Bioinformatics, Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan

年份：2013

卷号：128

起止页码：505

外文期刊名：Bioresource Technology

收录：EI(收录号：20124915764536)

语种：英文

外文关键词：Batch cell culture

摘要：Fed-batch cultures of Klebsiella pneumoniae expressing Escherichia coli aldH were performed under microaerobic conditions to investigate the effects on metabolites production. Increasing the aeration rate enhanced cell growth and 3-hydroxypropionic acid (3-HP) production, but reduced 1,3-propanediol (1,3-PDO) formation. The recombinant strain K. pneumoniae/pUC18. kan-aldHec produced 48.9. g/L of 3-HP and 25.3. g/L of 1,3-PDO with an overall yield of 0.66. mol/mol in 28. h at an aeration rate of 1.5. vvm; however, under fully aerobic condition, no 3-HP and 1,3-PDO were produced due to the repression of dha operon. The flux through the reaction catalyzed by glycerol dehydratase and the split ratio of 1,3-PDO were negatively correlated with the aeration rate, even though the 3-HP level showed a positive trend. This study demonstrated that the relative amounts of 3-HP and 1,3-PDO can be controlled by the aeration rate. ? 2012 Elsevier Ltd.