文献类型：期刊文献

英文题名：Functionalization of cubic Ia3d mesoporous silica for immobilization of penicillin G acylase

作者：Lü, Yongjun[1]; Lu, Guanzhong[1]; Wang, Yanqin[1]; Guo, Yanglong[1]; Guo, Yun[1]; Zhang, Zhigang[1]; Wang, Yunsong[1]; Liu, Xiaohui[1]

机构：[1] Lab. for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, China

年份：2007

卷号：17

期号：13

起止页码：2160

外文期刊名：Advanced Functional Materials

收录：EI(收录号：20073810816635)

语种：英文

外文关键词：Enzyme immobilization - Fourier transform infrared spectroscopy - Functional analysis - Hydrolysis - Nuclear magnetic resonance spectroscopy - Silica - Thermogravimetric analysis - Transmission electron microscopy - X ray diffraction

摘要：Functionalized cubic Ia3d mesoporous silica (FCIMS) with glycidoxypropyl groups was prepared and characterized by powder XRD, N2 adsorption, TGA, FT-IR spectroscopy, solid state 13C NMR spectroscopy and TEM, and studied as the support for the immobilization of Penicillin G acylase (PGA). The results show that the glycidoxypropyl groups have been chemically bonded to the silicon atoms on the surface of cubic Ia3d mesoporous silica (CIMS). The FCIMS materials possess the mesoporous structure with pore diameter of 8 nm which is ～1 nm less than that of CIMS (9 nm). The influence of the amount of glycidoxypropyl groups on the initial specific activity and operational stability of PGA immobilized on FCIMS were examined for the hydrolysis of penicillin G potassium salt. The results show that PGA has been successfully immobilized covalently on the FCIMS materials, and the initial specific activity of PGA/FCIMS is 835 IUg-1 and PGA/CIMS is 624 IUg-1. After repeated use for 10 times, PGA/FCIMS retains 72% of its initial specific activity and PGA/CIMS retains only 58%. ? 2007 WILEY-VCH Verlag GmbH & Co. KGaA.