文献类型：期刊文献

英文题名：Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms for Hydrogen Peroxide Sensing

作者：Li, Meng[1];Wu, Jing[1];Su, Haiping[1];Tu, Yan[1];Shang, Yazhuo[1];He, Yifan[2];Liu, Honglai[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Beijing Technol & Business Univ, Sch Sci, Dept Biotechnol, Beijing 100048, Peoples R China

年份：2019

卷号：19

期号：3

外文期刊名：SENSORS

收录：;EI(收录号：20191106617921);WOS:【SCI-EXPANDED(收录号:WOS:000459941200203)】；

基金：This work is supported by the National Natural Science Foundation of China (Project No. 21476072) and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：ionic liquids (ILs); polypyrrole (PPy); gold particles; horseradish peroxidase (HRP); enzyme immobilization; hydrogen peroxide sensor

摘要：In this work, three different aqueous solutions containing imidazole-based ILs with different alkyl chain lengths ([C(n)mim]Br, n = 2, 6, 12) were adopted as the medium for the synthesis of ionic liquid-polypyrrole (IL-PPy) composites. Herein, the ILs undertook the roles of the pyrrole solvent, the media for emulsion polymerization of PPy and PPy dopants, respectively. The electrochemical performances of the three IL-PPy composites on a glassy carbon electrode (GCE) were investigated by electrochemical experiments, which indicated that [C(12)mim]Br-PPy (C-12-PPy) composites displayed better electrochemical performance due to their larger surface area and firmer immobilization on the GCE. Further, C-12-PPy/GCE were decorated with Au microparticles by electrodeposition that can not only increase the conductivity, but also immobilize sufficient biomolecules on the electrode. Then, the obtained C-12-PPy-Au/GCE with outstanding electrochemical performance was employed as a horseradish peroxidase (HRP) immobilization platform to fabricate a novel C-12-PPy-Au-HRP/GCE biosensor for H2O2 detection. The results showed that the prepared C-12-PPy-Au-HRP/GCE biosensor exhibited high sensitivity, fast response, and a wide detection range as well as low detection limit towards H2O2. This work not only provides an outstanding biomolecule immobilization matrix for the fabrication of highly sensitive biosensors, but also advances the understanding of the roles of ILs in improving the electrochemical performance of biosensors.