文献类型：期刊文献

英文题名：Adsorption Characteristics and Enrichment of Emodin from Marine-Derived Aspergillus flavipes HN4-13 Extract by Macroporous Resin XAD-16

作者：Gong, Lizhi[1,2];Wu, Yuzhen[2];Qiu, Xiaohan[2];Xin, Xiujuan[2];An, Faliang[2];Guo, Miaomiao[1]

机构：[1]Beijing Technol & Business Univ, Key Lab Cosmet, China Natl Light Ind, 11-33 Fucheng Rd, Beijing 100048, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：20

期号：4

外文期刊名：MARINE DRUGS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000785183000001)】；

基金：This work was funded by the National Key R&D Program of China (2018YFC1706206), the National Natural Science Foundation of China (41876189), and the Open Research Fund Program of Key Laboratory of Cosmetic (Beijing Technology and Business University), China National Light Industry (KLC-2019-ZD2).

语种：英文

外文关键词：Aspergillus flavipes HN4-13; emodin; macroporous resin XAD-16; adsorption kinetics and isotherms; enrichment

摘要：Emodin, a hydroxyanthraquinone derivative, has been used as medicine for more than 2000 years due to its extensive pharmacological activities. Large-scale production of emodin has been achieved by optimizing the fermentation conditions of marine-derived Aspergillus flavus HN4-13 in a previous study. However, the fermentation broth contained complex unknown components, which adversely affected the study of emodin. Herein, the conditions for the enrichment of emodin from A. flavipes HN4-13 extract using XAD-16 resin were optimized, and a separation method with high efficiency, simple operation, a low cost, and a large preparative scale was established. The adsorption process of emodin on the XAD-16 resin conformed to pseudo-second-order kinetics and Langmuir models. The optimal conditions for the adsorption process were as follows: An emodin concentration, flow rate, and loading volume of 0.112 mg/mL, 2 BV/h, and 10 BV, respectively. For desorption, 50% ethanol was used to elute impurities and 80% ethanol was used to desorb emodin. After enrichment with XAD-16 resin, the emodin content increased from 1.16% to 11.48%, and the recovery rate was 75.53% after one-step treatment. These results demonstrate the efficiency of the simple adsorption-desorption strategy, using the XAD-16 resin for emodin enrichment.