文献类型：期刊文献

英文题名：Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae

作者：Huang, Yanna[1]; Li, Zhimin[1]; Shimizu, Kazuyuki[2]; Ye, Qin[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Bioscience and Bioinformatics, Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan

年份：2012

卷号：103

期号：1

起止页码：351

外文期刊名：Bioresource Technology

收录：EI(收录号：20115014595876)

语种：英文

外文关键词：Escherichia coli - Batch cell culture - Aldehydes - Cloning - Gene expression

摘要：In this study, an aldehyde dehydrogenase (ALDH) was over-expressed in Klebsiella pneumoniae for simultaneous production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO). Various genes encoding ALDH were cloned and expressed in K. pneumoniae, and expression of Escherichia coli aldH resulted in the highest 3-HP titer in anaerobic cultures in shake flasks. Anaerobic fed-batch culture of this recombinant strain was further performed in a 5-L reactor. The 3-HP concentration and yield reached 24.4g/L and 0.18mol/mol glycerol, respectively, and at the same time 1,3-PDO achieved 49.3g/L with a yield of 0.43mol/mol in 24h. The overall yield of 3-HP plus 1,3-PDO was 0.61mol/mol. Over-expression of the E. coli AldH also reduced the yields of by-products except for lactate. This study demonstrated the possibility of simultaneous production of 3-HP and 1,3-PDO by K. pneumoniae under anaerobic conditions without supply of vitamin B12. ? 2011 Elsevier Ltd.