文献类型：期刊文献

中文题名：菲的微生物酶促降解研究

英文题名：Study on Microbial Enzymatic Degradation of Phenanthrene

作者：陈继章[1];张卫[1];施倬嘉[1];乔建江[1];徐圣友[1];张大年[1]

机构：[1]华东理工大学资源与环境工程学院,上海200237

年份：2008

卷号：27

期号：4

起止页码：1623

中文期刊名：农业环境科学学报

外文期刊名：Journal of Agro-Environment Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(20677015);国家高技术研究发展计划(2007AA06Z300);国家科技部世博专项(2005BA908B16);中国博士后科学基金(20070420094)

语种：中文

中文关键词：菲;酶促降解;产酶条件;酶性质

外文关键词：phenanthrene; enzymatic degradation; enzyme production condition; enzyme property

摘要：微生物降解是环境中菲去除的重要途径。为有效开展菲污染的微生物酶学治理,采用室内模拟的方法,从菲污染土壤中分离到一株高效降解菌株,研究了其最适产酶条件。结果表明该菌株最适产酶条件为培养温度35℃,培养液起始pH7.0,培养时间60h,而Hg2+对该菌株产酶有显著抑制作用。从该优势菌中提取的粗酶液在pH7.0和30℃时显示最大的降解活性,其米氏常数为50.30nmol·mL-1,最大降解速率为171.33nmol·min-1·mg-1。
Phenanthrene is one of most important PAHs, and the US Environmental Protection Agency has listed phenanthrene as priority pollutant for remediation. Therefore, it is essential and urgent to remove phenanthrene from the environment quickly and effectively, phenanthrene could be removed by many processes, including volatilization, photooxidation, chemical oxidation, bioaccumulation, and adsorption in the environment. The principle process for the successful removal and elimination of phenanthrene from contaminated environment is microbial transformation and degradation. In order to effectively carry out removal of phenanthrene, microbial enzymatic degradation of phenanthrene was studied by laboratory simulation. A bacterium which can effectively degrade phenanthrene was isolated from soil contaminated by phenanthrene. The optimal temperature, initial pH value and culture time for enzyme production were 35 ℃, 7.0, 60 h, respectively, and Hg^2＋ can distinctly inhibit enzyme production. The optimal pH value and temperature for enzymatic degradation were 7.0 and 30℃. The Michaelis-Mentn＇ s constant, maximum reaction rate and degradation rate of the crude enzyme extracted from the dominant bacterium were 50.30 nmol·mL^-1, 5.14 nmol·min^-1 and 171.33 nmol·min^-1·mg^-1.