文献类型：期刊文献

英文题名：Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster

作者：Yang, Xue-Peng[1];Zhong, Gui-Fang[1];Lin, Jin-Ping[2];Mao, Duo-Bin[1];Wei, Dong-Zhi[1,2]

机构：[1]Zhengzhou Univ Light Ind, Sch Food & Biol Engn, Zhengzhou 450002, Peoples R China;[2]E China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：37

期号：6

起止页码：575

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242716579089);WOS:【SCI-EXPANDED(收录号:WOS:000277789300004)】；

基金：This work was supported by the National Natural Science Foundation of China (grant No. 20976053), and by the National Basic Research Program (973) of China (grant No. 2009CB724703).

语种：英文

外文关键词：Biosynthesis; Expression; Gene cluster; Pyrroloquinoline quinone

摘要：We have expressed the pqqABCDE gene cluster from Gluconobacter oxydans, which is involved in pyrroloquinoline quinone (PQQ) biosynthesis, in Escherichia coli, resulting in PQQ accumulation in the medium. Since the gene cluster does not include the tldD gene needed for PQQ production, this result suggests that the E. coli tldD gene, which shows high homology to the G. oxydans tldD gene, carries out that function. The synthesis of PQQ activated d-glucose dehydrogenase in E. coli and the growth of the recombinant was improved. In an attempt to increase the production of PQQ, which acts as a vitamin or growth factor, we transformed E. coli with various recombinant plasmids, resulting in the overproduction of the PQQ synthesis enzymes and, consequently, PQQ accumulation-up to 6 mM-in the medium. This yield is 21.5-fold higher than that obtained in previous studies.