文献类型：期刊文献

英文题名：ENFET glucose biosensor produced with mesoporous silica microspheres

作者：Yao, Ke[1]; Zhu, Yihua[1]; Wang, Ping[1]; Yang, Xiaoling[1]; Cheng, Pengzhi[1]; Lu, Hui[2]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Phys, Shanghai 200237, Peoples R China

年份：2007

卷号：27

期号：4

起止页码：736

外文期刊名：MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS

收录：;EI(收录号：20071710570435);WOS:【SCI-EXPANDED(收录号:WOS:000246630600021)】；

语种：英文

外文关键词：ENFET; glucose; mesoporous silica microspheres; biosensor

摘要：The biosensors based on ENFETs for direct glucose oxidase (GOD) concentration analysis have been fabricated. Mesoporous silica (MS) microspheres of different pore sizes were used as the substrates for immobilizing enzyme to construct ENFETs. The MS microspheres show significantly improved enzyme immobilization capacity. The glucose sensitive ENFET based on the modification of the gate surface of ion-sensitive field-effect transistors (ISFETs) with MS microspheres and glucose oxidase (GOD) shows obviously enhanced sensitivity and extended lifetime compared with the conventional ones. MS microspheres of different pore sizes were investigated and the sensor with remodeled MS microspheres which experienced the pore expanding treatment show better performance than untreated MS microspheres. The fabricated sensors have a linear range of 0.25-2.0 mM, and a detection limit of ca. 0.10 mM. The influence of buffer concentration, ionic strength and pH was discussed. The biosensor also has good stability and reproducibility. (C) 2006 Elsevier B.V. All rights reserved.