文献类型：期刊文献

英文题名：Long-circulation of hemoglobin-loaded polymeric nanoparticles as oxygen carriers with modulated surface charges

作者：Xu, Feng[1,2];Yuan, Yuan[1,2];Shan, Xiaoqian[1,2];Liu, Changsheng[1,2];Tao, Xinyi[3];Sheng, Yan[1,2];Zhou, Huanjun[1,2]

机构：[1]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2009

卷号：377

期号：1-2

起止页码：199

外文期刊名：INTERNATIONAL JOURNAL OF PHARMACEUTICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000268471300027)】；

基金：The authors acknowledge the financial support from the National High Technology Research and Development Program of China (863 program) (No. 2004AA-302050) and from Shanghai Nanotechnology Special Foundation (No. 0452nm022).

语种：英文

外文关键词：Hb-loaded polymeric nanoparticles; Blood long-circulation; zeta-Potential; Biodistribution

摘要：The aim of this study was to investigate the effects of the surface charges on the in vitro macrophage cellular uptake and in vivo blood clearance and biodistribution of the hemoglobin-loaded polymeric nanoparticles (HbPNPs). The surface charges of the HbPNPs fabricated from mPEG-PLA-mPEG were modulated with cationized cetyltrimethylammonium bromide (CTAB) and anionized sodium dodecyl sulphate (SIDS), respectively. In vitro macrophage cellular uptake and in vivo biodistribution of the coumarin 6-labeled HbPNPs with different electric charges were investigated, and the half-lives in the circulation were pharmacokinetically analyzed. The particle sizes of the HbPNPs were all below 200 nm with a narrow size distribution and high encapsulation efficiency (>84%). And the zeta-potentials of the untreated, cationized and anionized HbPNPs in phosphate buffered sodium chloride solution (PBS) were -12.3, +3.28 and -25.4mV, respectively. The HbPNPs did not occur significant aggregation or sedimentation, even after 5 days. Compared with the untreated HbPNPs. 1-fold decrease/increase of the uptake percentage associated with the cationized/anionized HbPNPs was observed. In vivo experiment demonstrated that the calculated half-life of the cationized HbPNPs was 10.991 h, 8-fold longer than that of the untreated HbPNPs (1.198 h). But the anionized HbPNPs displayed opposite effect. Furthermore, the cationized HbPNPs mainly accumulated in the liver, lung and spleen after 48 h injection. MTT results showed that the HbPNPs with different surface charges all exhibited slight toxicity. These results demonstrated that the CPAB-modulated HbPNPs with low positive charge and suitable size have a promising potential as a long-circulating oxygen carrier system with desirable biocompatibility and biofunctionality. (C) 2009 Elsevier B.V. All rights reserved.