文献类型：专利

英文题名：Serum-free culture medium useful for in vitro amplification of human immune killer cell comprises carbohydrate, amino acids, lipids, nucleosides, vitamins, salts, hormones, protein and hydroxyethyl piperazineethanesulfonic acid and phenol

作者：CAI H;TAN W;CHEN X;ZHOU Y

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN106834229-A

申请日：2017-01-25

公开日：2017-06-13

语种：英文

收录：DERWENT

摘要：NOVELTY - Serum-free culture medium comprises carbohydrate, amino acids, lipids, nucleosides, vitamins, salts, hormones, protein and hydroxyethyl piperazineethanesulfonic acid and phenol. The carbohydrate comprises D-glucose, sodium pyruvate. The amino acid comprises e.g. L-alanine, L-arginine and hydrochloric acid, L-asparagine water, L-aspartic acid, L-cysteine hydrochloride water, L-cystine dihydrochloride, L-sodium glutamate, L-glutamine glycine, L-histidine hydrochloride water, L-isoleucine, L-leucine, L-lysine-hydrochloride, L-methionine, L-proline, L-serine, L-valine and para aminobenzoic acid. USE - The serum-free culture medium is useful for in vitro amplification of human immune killer cell (claimed). ADVANTAGE - The serum-free culture medium is clear, has high efficiency and stability, promotes the biological research and immune cell therapy. DETAILED DESCRIPTION - Serum-free culture medium comprises carbohydrate, amino acids, lipids, nucleosides, vitamins, salts, hormones, protein and hydroxyethyl piperazineethanesulfonic acid and phenol. The carbohydrate comprises D-glucose, sodium pyruvate. The amino acid comprises L-alanine, L-arginine and hydrochloric acid, L-asparagine water, L-aspartic acid, L-cysteine hydrochloride water, L-cystine dihydrochloride, L-sodium glutamate, L-glutamine glycine, L-histidine hydrochloride water, L-isoleucine, L-leucine, L-lysine-hydrochloride, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine disodium dihydrate, L-valine and para aminobenzoic acid. The lipids include linoleic acid, choline chloride, ethanolamine, alpha -thioglycerol, linolenic acid and cholesterol. The nucleoside comprises sodium hypoxanthine and thymidine. The vitamin comprises D-biotin, folic acid, myo-inositol, niacinamide, calcium pantothenate, pyridoxine hydrochloride, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, cyanocobalamin and lipoic acid. The salt comprises calcium chloride, calcium nitrate, potassium chloride, potassium nitrate, disodium hydrogen phosphate, sodium chloride, sodium dihydrogen phosphate monohydrate, magnesium sulfate, magnesium chloride, sodium bicarbonate, sodium selenite, ferric nitrate nonahydrate, heptahydrate ferrous sulfate, penta hydrated copper sulfate, heptahydrate zinc sulfate, beta -glycerol disodium phosphate, ammonium iron citrate and putrescine. The hormone comprises insulin. The protein comprises bovine serum albumin and transferring.