文献类型：期刊文献

中文题名：铁皮石斛多糖的初级结构分析

英文题名：Primary Structural Analysis of Dendrobium officinale Polysaccharide

作者：高云霄[1];胡小龙[1];王月荣[1];姜志宏[2];章弘扬[1];张敏[3];胡坪[1]

机构：[1]上海市功能性材料化学重点实验室华东理工大学化学与分子工程学院,上海200237;[2]澳门科技大学中医药学院,澳门999078;[3]上海市新药设计重点实验室华东理工大学药学院,上海200237

年份：2018

卷号：39

期号：5

起止页码：934

中文期刊名：高等学校化学学报

外文期刊名：Chemical Journal of Chinese Universities

收录：CSTPCD;;EI(收录号：20183805830851);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000435335700014)】；北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：Supported by the State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, China (No. MUST-SKL-2016-06).

语种：中文

中文关键词：铁皮石斛;多糖;结构分析;核磁共振

外文关键词：Dendrobium officinale; Polysaccharide; Structure analysis; Nuclear magnetic resonance

摘要：从新鲜的铁皮石斛中分离得到一种均一的铁皮石斛多糖(SDOP),分别采用高效凝胶色谱法(GPC)法和1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化法测定其重均分子量和单糖组成,并通过红外光谱、甲基化分析和核磁共振波谱对其初级结构进行分析.结果表明,SDOP的重均分子量为1.66×106,含有甘露糖和葡萄糖(摩尔比4.9∶1.0).通过甲基化和核磁共振波谱分析结果推测,SDOP是一种以1,4-链接为主,存在少量1,2,4-,1,3,4-,1,4,6-分支结构和端基结构的O-乙酰化葡甘露聚糖,O-乙酰基主要连接在糖环的2号和3号位.
A homogeneous polysaccharide（ SDOP） was obtained from the fresh Dendrobium officinale. The relative molecular mass and composition of monosaccharide were then determined by gel permeation chromatography-laser scatter（ GPC-LS） and high performance liquid chromatography with pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone（ PMP） as derivatization agent. The detailed chemical structure of SDOP was elucidated by means of infrared spectroscopy,methylation analysis and nuclear magnetic resonance spectroscopy（ NMR）. The result shows that SDOP with an Mwof 1. 66×10~6 consists dominantly of mannose and glucose in a molar ratio of 4. 9 ∶ 1. 0. The results of NMR and methylation analysis suggest that SDOP is a 1,4-linked O-acetylated glucomannan,with a small amount of branch and end signal,while acetyl groups are mainly attached to the O-2 or O-3 positions on saccharide ring.