文献类型：期刊文献

英文题名：ATP and ssDNA aptamer-mediated peroxidase-like activity of rGO@PDA@CeO2 nanozyme: Exosomal proteins profiling and detection at physiological pH for colorimetric sensor

作者：Kuang, Jingjing[1];Ruan, Shengli[1];Sun, Yangkun[1];Wu, Zeyu[1];Xu, Jiayao[1];Zhang, Ting[1];You, Xiaofan[1];Yang, Shiman[1];Zhang, Min[2];Zhang, Hongyang[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Techn Cell M, Sch Pharm, Shanghai 200237, Peoples R China

年份：2023

卷号：394

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20233314547181);WOS:【SCI-EXPANDED(收录号:WOS:001327470500001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant number 81973285).

语种：英文

外文关键词：RGO@PDA@CeO2; ATP; SsDNA aptamer; Exosome; Colorimetric sensor

摘要：Exosomes are extracellular esicles that inherit molecular markers from their parent cells for the transfer and deliery of membrane and cytosolic molecules between cells. Exosome detection and protein profiling proide noninasie access to disease diagnosis and treatment. The nanozyme designed in this paper contains polydopamine-functionalization of graphene oxide (rGO@PDA) units that can adsorb ssDNA aptamers and CeO2 metal oxide nanosheet units with peroxidase actiity. As auxiliary regulator, adenosine triphosphate (ATP) enhances peroxidase actiity of rGO@PDA@CeO2 under a wide range of pH (4-7.4), which breaks through the limitation that traditional nanozymes can only exhibit peroxidase actiity under acidic conditions. The ssDNA aptamers, which are recognition probes of exosome, can be adsorbed on the surface of rGO@PDA by aromatic stacking, competing with ATP molecules. Thus, the ssDNA aptamer acts as the switch for colorimetric sensor to flexibly regulate the peroxidase actiity of the rGO@PDA@CeO2 under physiological conditions, and realizes sensitie detection of exosomes. This assay achiees a detection linear range of 0.46 x 10(7)-10(8) particle/mL and a limit down to 3.81 x 10(5) particles/mL. In addition, this aptasensor sensitiely profiles six exosomal protein across fie cell types and clinical samples, suggesting their potential in clinic diagnosis.