文献类型：期刊文献

英文题名：Construction of a hierarchical micro & nanoporous surface for loading genistein on the composite of polyetheretherketone/tantalum pentoxide possessing antibacterial activity and accelerated osteointegration

作者：Mei, Shiqi[1];Wang, Fan[1];Hu, Xinglong[1];Yang, Kong[2];Xie, Dong[3];Yang, Lili[3];Wu, Zhaoying[4];Wei, Jie[1]

机构：[1]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]Southwest Minzu Univ, Inst Qinghai Tibetan Plateau, Chengdu 610064, Peoples R China;[3]Second Mil Med Univ, Changzheng Hosp, Dept Orthopaed Surg, Shanghai 200003, Peoples R China;[4]Sun Yat Sen Univ, Sch Biomed Engn, Guangzhou 510006, Guangdong, Peoples R China

年份：2021

卷号：9

期号：1

起止页码：167

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20210209763355);WOS:【SCI-EXPANDED(收录号:WOS:000607370000011)】；

基金：The grants were from the Shanghai Science and Technology Development Foundation (17441900600 and 19441906100), the National Natural Science Foundation of China (81771990 and 81801845), and Shenzhen Fundamental Research Program (JCYJ20190807160811355).

语种：英文

外文关键词：Controlled drug delivery - Tantalum oxides - Drug products - Flavonoids - Bone - Escherichia coli - Stem cells - Cell culture - Targeted drug delivery

摘要：Nanoporous tantalum pentoxide (NTP) particles with a pore size of about 10 nm were synthesized and blended with polyetheretherketone (PEEK) to fabricate a PEEK/NTP composite (PN). Subsequently, PN was treated by concentrated sulfuric acid to create a microporous surface (pore size of around 2 mu m) on sulfonated PN (SPN), which formed a hierarchical micro & nanoporous surface. Compared with PN, the porous surface of SPN exhibited higher roughness, hydrophilicity, and surface energy. In addition, genistein (GT) was loaded into the porous surface of SPN (SPNG), which showed high GT loading capacity and sustained release of GT into phosphate buffered saline (PBS). Moreover, SPNG revealed excellent antibacterial activity, which inhibited bacterial (E. coli and S. aureus) growth in vitro due to the synergistic effects of both sulfonic acid (SO3H) groups and the sustained release of GT. Compared with PN, SPN significantly improved the adhesion, proliferation, and osteogenic differentiation of bone mesenchymal stem cells in vitro. Moreover, compared with SPN, SPNG further enhances the cell responses. Compared with PN, SPN remarkably improved bone formation and osteointegration in vivo. Furthermore, compared with SPN, SPNG further enhanced the osteointegration. In short, SPNG with a micro & nanoporous surface, SO3H groups, and the sustained release of GT exhibited antibacterial activity and accelerated osteointegration, which would have tremendous potential as drug-loaded implants for bone substitute.