文献类型：期刊文献

中文题名：一种甲酯化n-C16-地衣素的分离及结构鉴定

英文题名：Isolation and Structural Characterization of Esterified n-C16-lichenysin with Methanol

作者：李艺明[1];杨世忠[1];牟伯中[1]

机构：[1]华东理工大学应用化学研究所,上海200237

年份：2009

卷号：72

期号：11

起止页码：1008

中文期刊名：化学通报

外文期刊名：Chemistry

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目(50744016);上海市国际合作项目(071607014)资助

语种：中文

中文关键词：脂肽;地衣素;分离;结构;地衣芽胞杆菌

外文关键词：Lipopeptide, Lichenysin, Separation, Structrural characterization, Bacillus licheniformis

摘要：经过酸沉淀分离、有机溶剂萃取和二步柱分离,从地衣芽胞杆菌(Bacillus licheniformis)HSN221菌株的培养液中分离纯化得到了一种地衣素类似物。经ESI-MS测定,该物质的分子量为1062Da;经对酸解后甲醇酯化的脂肪酸的GC/MS分析可知,该物质含有3-羟基正十六烷酸残基;Q-TOFMS/MS的结果显示该物质具有GlnLeuLeuValAspMeLeuIle的肽链,因此,该物质为甲酯化的n-C16-地衣素。
The lipopeptide produced by Bacillus licheniforrnis HSN 221 was separated from the cell-free broth by the methods of acid precipitating, solvent extracting and purified by two-step column separation. Its molecular structure was determined by ESI-MS, GC/MS and Q-TOF MS/MS. The results showed that the lipopeptide with MW of 1062Da contains 3- hydroxylhexadecoic acid and peptide chain of GlnLeuLeuValAspMeLeulle, which indicates that it is an esterified n-C16- liehenysin with methanol.