文献类型：专利

英文题名：Purifying erythromycin A from fermentation broth comprises e.g. taking canned erythromycin fermentation broth, filtering, regulating pH, performing fixed-bed resin absorption, washing non-polar macroporous absorption resin, and eluting

作者：WU B;ZHU J;JI L;CHEN K;WU Y;ZHU S

机构：[1]UNIV EAST CHINA SCI&TECHNOLOGY

申请号：CN102180922-A

申请日：2011-03-18

公开日：2011-09-14

语种：英文

收录：DERWENT

摘要：NOVELTY - Purifying erythromycin A from a fermentation broth comprises (i) taking canned erythromycin fermentation broth, filtering, regulating pH of the filtrate, performing fixed-bed resin absorption, and controlling erythromycin absorption amount, (ii) using borax-sodium hydroxide buffer, or an organic solvent as a washing agent to wash a non-polar macroporous absorption resin with the target in the step (i), (iii) loading the washed non-polar macroporous absorption resin in a column and eluting with 1BV deionized water, and then with butyl acetate, and (iv) crystallizing erythromycin A, and drying. USE - The method is useful for purifying erythromycin A from the fermentation broth. ADVANTAGE - The method solves problem of low elution ratio of 1BV erythromycin, high amount of impurities in products, and poor resin regeneration effect as compared to the existing resin absorption separating methods. DETAILED DESCRIPTION - Purifying erythromycin A from a fermentation broth comprises (i) taking canned erythromycin fermentation broth as a raw material, filtering using a membrane, regulating pH of the filtrate to 9-10, performing fixed-bed resin absorption, and controlling erythromycin absorption amount to 60-70% of the resin saturated absorption amount, (ii) using borax-sodium hydroxide buffer (pH 8-11) with tetraborate ions (0.05 mol/l), or an organic solvent as a washing agent, which is completely or partially miscible in water, washing a non-polar macroporous absorption resin with the target in the step (i) at 40 degrees C, and terminating washing when the pH of an instantaneous effluent liquid at an outlet of the fixed-bed becomes same as that of the washing agent, (iii) loading the washed non-polar macroporous absorption resin obtained from the step (ii) in a column and eluting with 1BV deionized water, and then eluting with butyl acetate as eluent, and (iv) crystallizing erythromycin A from the first 1BV eluate, and drying to obtain the target product, where the non-polar macroporous absorption resin is Amberlite XAD-16 (RTM: Polymeric adsorbent resin), Amberlite XAD-1600 (RTM: Polymeric adsorbent resin), Sepabeads SP207 (RTM: Synthetic adsorbents) or Sepabeads SP825 (RTM: Synthetic adsorbents).