文献类型：期刊文献

英文题名：Enhancement and orchestration of osteogenesis and angiogenesis by a dual-modular design of growth factors delivery scaffolds and 26SCS decoration

作者：Tang, Wei[1];Yu, Yuanman[2];Wang, Jing[2];Liv, Hui[1];Pan, Haobo[1];Wang, Guocheng[1];Liu, Changsheng[2]

机构：[1]Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Res Ctr Human Tissues & Organs Degenerat, Shenzhen 518055, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2020

卷号：232

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20195107877564);WOS:【SCI-EXPANDED(收录号:WOS:000514748200039)】；

基金：The authors would like to express their gratitude to the financial supports from the National Key R&D Program of China (2018YFC1105201), National Natural Science Foundation of China (31700835, 51872318), Shenzhen Science and Technology Research Funding (JCYJ20170413161800287, JCYJ20160531171344016 and JCYJ20170413162104773).

语种：英文

外文关键词：Bone regeneration; Mesoporous bioactive glass; 2-N,6-O-sulfated chitosan; Dual-modular; Growth factor

摘要：Preserving the bioactivity of growth factors (GFs) and mimicking their in vivo supply patterns are challenging in the development of GFs-based bone grafts. In this study, we develop a 2-N, 6-O-sulfated chitosan (26SCS) functionalized dual-modular scaffold composed of mesoporous bioactive glass (MBG) with hierarchical porous structures (module I) and GelMA hydrogel columns (module II) in situ fixed in hollowed channels of the module I, which is capable of realizing differentiated delivery modes for osteogenic rhBMP-2 and angiogenic VEGF. A combinational release profile consisting of a high concentration of VEGF initially followed by a decreasing concentration over time, and a slower/sustainable release of rhBMP-2 is realized by immobilizing rhBMP-2 in module I and embedding VEGF in module II. Systematic in vitro and in vivo studies prove that the two coupled processes of osteogenesis and angiogenesis are well-orchestrated and both enhanced ascribed to the specific GFs delivery modes and 26SCS decoration. 26SCS not only enhances the GFs' bioactivity but also decreases antagonism effects of noggin. This study highlights the importance of differentiating the delivery pattern of different GFs and likely sheds light on the future design of growth factor-based bone grafts.