文献类型：期刊文献

英文题名：Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase

作者：Chu, Zhanying[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：1036

起止页码：179

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20182505335137);WOS:【SCI-EXPANDED(收录号:WOS:000445197900021)】；

基金：We gratefully acknowledged the support of the National Natural Science Foundation of China (No. 21475044), the National Key Technology R&D Program (2015BAK44B00), the National Key Scientific Instrument and Equipment Development Project (No. 2012YQ004403) and the Fundamental Research Funds for the Central Universities (222201817022).

语种：英文

外文关键词：Polymer-silica composite stationary phase; In-situ cross-linking polymerization; Mixed-mode separation; High performance liquid chromatography

摘要：A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase. The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction. The porous spherical silica (4 mu m diameter) with 300 A pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability. The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase. Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively. Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups. The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2). Finally, the stationary phase was applied to the separation of ginseng extract. (c) 2018 Elsevier B.V. All rights reserved.