文献类型：期刊文献

中文题名：A Novel and Simple Molecular Probe Sensing Hg（II） in Living Cells by Chelation-enhanced Fluorescence

英文题名：A Novel and Simple Molecular Probe Sensing Hg(II) in Living Cells by Chelation-enhanced Fluorescence

作者：Meng Qinghua[1];Cheng Sen[1];Lan Minbo[2];Wei Gang[3]

机构：[1]Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]CSIRO Mat Sci & Engn, Lindfield, NSW 2070, Australia

年份：2012

卷号：30

期号：2

起止页码：249

中文期刊名：Chinese Journal of Chemistry

外文期刊名：CHINESE JOURNAL OF CHEMISTRY

收录：;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000300674000009)】；CSCD:【CSCD2011_2012】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (No. 0752nm016) and we thank Dr. Yanlong Wang and Wenjuan Yu (Instrumental Analysis Center, Shanghai Jiao Tong University) for the provision of the mass spectrometry service.

语种：英文

中文关键词：mercury;probe;semirigid;chelation-enhanced fluorescence （CHEF）;fluorescent image

外文关键词：mercury; probe; semirigid; chelation-enhanced fluorescence (CHEF); fluorescent image

摘要：Mercury is currently widely used in industries which leads to various means of Hg（II） waste exposure and its accumulation in organisms will cause neurological damages. Thus, there is a great need for the design of probes or sensors with high sensitivity and selectivity for detecting and monitoring Hg（II） at physiological pH. Thus a novel and simple molecular probe P1 was prepared from 1,1＇-（1,3-phenylene）-bis（2,4-pentanedionato） for sensing Hg（II） via chelation-enhanced fluorescence （CHEF） mechanism. The probe indicated a selectively fluorescent response to Hg（II） in aqueous media excited by the ultraviolet light of 254 nm. The recognition mechanism was further studied by semi-empirical AM1 and molecular mechanics MM＋ methods in HyperChem 8.0. The calculation indicated a tetrahedron coordination geometry for Hg（II） and a chair-like configuration for the total molecule. The fluorescent images sensing Hg（II） in living mouse fibroblast cells by the probe were obtained by fluorescence microscope.
Mercury is currently widely used in industries which leads to various means of Hg(II) waste exposure and its accumulation in organisms will cause neurological damages. Thus, there is a great need for the design of probes or sensors with high sensitivity and selectivity for detecting and monitoring Hg(II) at physiological pH. Thus a novel and simple molecular probe P1 was prepared from 1,1'-(1,3-phenylene)-bis(2,4-pentanedionato) for sensing Hg(II) via chelation-enhanced fluorescence (CHEF) mechanism. The probe indicated a selectively fluorescent response to Hg(II) in aqueous media excited by the ultraviolet light of 254 nm. The recognition mechanism was further studied by semi-empirical AM1 and molecular mechanics MM+ methods in HyperChem 8.0. The calculation indicated a tetrahedron coordination geometry for Hg(II) and a chair-like configuration for the total molecule. The fluorescent images sensing Hg(II) in living mouse fibroblast cells by the probe were obtained by fluorescence microscope.