文献类型：期刊文献

英文题名：Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens

作者：Liu, Shiyou[1,2];Wang, Lin[1];Zheng, Huajun[3];Xu, Zhixiao[1];Roellig, Dawn M.[2];Li, Na[1,2];Frace, Michael A.[4];Tang, Kevin[4];Arrowood, Michael J.[2];Moss, Delynn M.[2];Zhang, Longxian[5];Feng, Yaoyu[1];Xiao, Lihua[2]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Atlanta, GA 30333 USA;[3]Chinese Natl Human Genome Ctr, Key Lab Hlth & Dis Genom, Shanghai Minist Sci & Technol, Shanghai 201203, Peoples R China;[4]Ctr Dis Control & Prevent, Div Sci Resources, Atlanta, GA 30333 USA;[5]Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

年份：2016

卷号：17

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000375978900001)】；

基金：This work was supported by the National Natural Science Foundation of China (31425025 and 31229005), Open Funding Project of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou, China (SKLVEB2014KFKT008), and the US Centers for Disease Control and Prevention.

语种：英文

外文关键词：Cyclospora; Genomics; Genome; Genetics; Evolution; Apicomplexan

摘要：Background: Cyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. The investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. In this study we sequenced the genome of C. cayetanensis and inferred its metabolism and invasion components based on comparative genomic analysis. Results: The genome organization, metabolic capabilities and potential invasion mechanism of C. cayetanensis are very similar to those of Eimeria tenella. Propanoyl-CoA degradation, GPI anchor biosynthesis, and N-glycosylation are some apparent metabolic differences between C. cayetanensis and E. tenella. Unlike Eimeria spp., there are no active LTR-retrotransposons identified in C. cayetanensis. The similar repertoire of host cell invasion-related proteins possessed by all coccidia suggests that C. cayetanensis has an invasion process similar to the one in T. gondii and E. tenella. However, the significant reduction in the number of identifiable rhoptry protein kinases, phosphatases and serine protease inhibitors indicates that monoxenous coccidia, especially C. cayetanensis, have limited capabilities or use a different system to regulate host cell nuclear activities. C. cayetanensis does not possess any cluster of genes encoding the TA4-type SAG surface antigens seen in E. tenella, and may use a different family of surface antigens in initial host cell interactions. Conclusions: Our findings indicate that C. cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. Amino acid metabolism and post-translation modifications of proteins are some major differences between C. cayetanensis and other apicomplexans. The whole genome sequence data of C. cayetanensis improve our understanding of the biology and evolution of this major foodborne pathogen and facilitate the development of intervention measures and advanced diagnostic tools.