文献类型：期刊文献

英文题名：Diversity and distribution of sulfate-reducing bacteria in four petroleum reservoirs detected by using 16S rRNA and dsrAB genes

作者：Guan, Jing[1,2];Xia, Li-Ping[1,2];Wang, Li-Ying[1,2];Liu, Jin-Feng[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

年份：2013

卷号：76

起止页码：58

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000312759300011)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503).

语种：英文

外文关键词：Petroleum microbiology; Sulfate-reducing bacteria; Corrosion; CCA; 16S rRNA gene; dsrAB genes

摘要：Microbial sulfate reduction, an important metabolic process in petroleum reservoirs, is widely known as a major contributor to microbial influenced corrosion and deterioration of oil quality. To better control oil field corrosion and oil degradation caused by the sulfate-reducing bacteria (SRBs), the community structure and composition of SRBs in four oil reservoirs were investigated in this study by comparing clone libraries of 165 rRNA and dissimilatory sulfate reductase (dsrAB) genes. In addition, canonical correspondence analysis (CCA) was also employed to find relationship between biodata and physiochemical information. More information on SRB communities was obtained from nested-PCR-phylogenetic analyses of 165 rRNA genes and PCR primer sets amplifying six groups of SRBs frequently detected in oilfields all over the world were used. Amplified sequences belonging to Desulfotomaculum and Desulfobacter were the most dominant in all four reservoirs. The diversity of SRB communities increased while the temperature of the four oil reservoirs decreased from 63 to 21. Correlations between environmental variables and species distribution indicated that Desulfotomaculum was correlated with temperature, depth, and the concentration of acetate, propionate and sulphate. Desulfomicrobium. Desulfobacter and Desulfobulbus showed positive correlation with sulphur and salinity. Desulfobacterium was influenced by both salinity and the concentration of acetate. The results of this study provided important information on the microbial ecology of sulfate-reducing bacteria in different petroleum reservoirs. (C) 2012 Elsevier Ltd. All rights reserved.