文献类型：期刊文献

英文题名：A dual signal amplification method for exosome detection based on DNA dendrimer self-assembly

作者：Gao, Mei-Ling[1];He, Fang[1];Yin, Bin-Cheng[1];Ye, Bang-Ce[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Hangzhou 310014, Zhejiang, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Xinjiang 832000, Peoples R China

年份：2019

卷号：144

期号：6

起止页码：1995

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000460765600013)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (Grants 21822402, 21675052, 21335003, 21575089), the Fundamental Research Funds for the Central Universities, the Science Fund for Creative Research Groups (Grant 21421004), and the Programme of Introducing Talents of Discipline to University (Grant B16017).

语种：英文

摘要：An increasing number of studies have found that circulating exosomes play a vital role in the occurrence and metastasis of cancer. Therefore, a direct, sensitive and specific method for detection of tumor exosomes will contribute to the diagnosis and prognosis of cancer. In this work, we take advantage of the facile adaptability of aptamers to design an exosome quantitative method, which converts an exosome capture event to nucleic acid detection. With the help of a hairpin DNA cascade reaction (HDCR) and easy accessibility of DNA dendrimer self-assembly, dual signal amplification was achieved. A CD63 aptamer linked via a DNA probe to magnetic beads acts as the capture component. In the presence of target exosomes, aptamers identify and combine with exosomes, releasing the DNA probe as a trigger to initiate the HDCR (the first signal amplification process) by opening hairpin DNA (HP1) bound to gold nanoparticles (AuNPs). Fluorescently-labeled DNA dendrimers concatenate with HP1 as the second signal amplification stage to increase the signal-to-noise ratio. Under the optimal conditions, our method achieved a good linear response for HepG2 cell-derived exosomes in a concentration range from 1.75 x 10(3) to 7.0 x 10(6) particles per mu L with a detection limit of 1.16 x 10(3) particles per mu L. It also shows a good performance for detection of exosomes in biological samples.