文献类型：期刊文献

中文题名：乙二胺羟丙基壳聚糖固定化天门冬酰胺酶的研究

英文题名：Study on the Immobilization of L -asparaginase with Ethylenediamine-hydroxypropyl Chitosan

作者：方波[1];王任远[1];宋道云[1];周纪宁[1];江体乾[1];陈鸿雁[1]

机构：[1]华东理工大学化学工程研究所,上海200237

年份：2001

卷号：27

期号：4

起止页码：341

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目 (2 980 60 0 6)

语种：中文

中文关键词：天门冬酰胺酶;固定化酶;壳聚糖胺基衍生物;乙二胺羟丙基壳聚糖;活化载体;蛋白质合成

外文关键词：L asparaginase; immobilized enzyme; chitosan; aminated chitosan derivative

摘要：研制了新型壳聚糖胺基衍生物——乙二胺羟丙基壳聚糖 ( EDA- HPCS) ,并将其用于固定天门冬酰胺酶。分别考察了甲醛活化载体和戊二醛活化载体对固定化酶活力的影响。结果表明 ,在适当的固定化条件下 ,甲醛活化 EDA- HPCS的固定化酶活力约为 30 U/g载体 ,而戊二醛活化EDA- HPCS固定化酶活力约为 1
A novel aminated chitosan derivative, ethylenediamine hydroxypropyl chitosan (EDA HPCS), was prepared and was applied to immobilize L asparaginase. The effects of formadehyde activated and glutaraldehyde activated carriers on the activities of immobilized enzyme were investigated. The results showed that, at proper immobilization conditions, the activities of immobilized enzyme with formadehyde activated EDA HPCS were about 30 U/g and the activities of immobilized enzyme with glutaraldehyde activated EDA HPCS were about 16U/g.