文献类型：期刊文献

英文题名：The Parabrachial Nucleus Directly Channels Spinal Nociceptive Signals to the Intralaminar Thalamic Nuclei, but Not the Amygdala

作者：Deng, Juan[1];Zhou, Hua[1];Lin, Jun-Kai[1,2];Shen, Zi-Xuan[3];Chen, Wen-Zhen[1,2];Wang, Lin-Han[1,2];Li, Qing[1];Mu, Di[1];Wei, Yi-Chao[1];Xu, Xiao-Hong[1];Sun, Yan-Gang[1,4]

机构：[1]Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China;[2]Univ Chinese Acad Sci, 19A Yu Quan Rd, Beijing 100049, Peoples R China;[3]East China Univ Sci & Technol, Dept Biotechnol, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[4]Shanghai Ctr Brain Sci & Brain Inspired Intellige, Shanghai 201210, Peoples R China

年份：2020

卷号：107

期号：5

起止页码：909

外文期刊名：NEURON

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000569855600002)】；

基金：We thank Yan-Jing Zhu, Xiao-Jun Chen, and Lei Yuan for technical support and all the members of the Yan-Gang Sun laboratory for helpful discussion. We thank Dr. Qian Hu and the optical imaging core facility for their assistance in imaging and data analysis. We thank Dr. Xiang Yu for providing the AAV2/8-hSyn-DIO-synaptophysin-tdTomato construct and Dr. Miao He for providing the H2B-GFP mice. This work is supported by the National Natural Science Foundation of China (grants 61890952 and 31825013), the Shanghai Municipal Science and Technology Major Project (grant 2018SHZDZX05), and the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB32010200). J.D. is supported by the National Postdoctoral Program for Innovative Talents of China (grant BX201800340), the Shanghai Post-doctoral Excellence Program (grant 2018032), and the SA-SIBS Scholarship Program.

语种：英文

摘要：The parabrachial nucleus (PBN) is one of the major targets of spinal projection neurons and plays important roles in pain. However, the architecture of the spinoparabrachial pathway underlying its functional role in nociceptive information processing remains elusive. Here, we report that the PBN directly relays nociceptive signals from the spinal cord to the intralaminar thalamic nuclei (ILN). We demonstrate that the spinal cord connects with the PBN in a bilateral manner and that the ipsilateral spinoparabrachial pathway is critical for nocifensive behavior. We identify Tacr1-expressing neurons as the major neuronal subtype in the PBN that receives direct spinal input and show that these neurons are critical for processing nociceptive information. Furthermore, PBN neurons receiving spinal input form functional monosynaptic excitatory connections with neurons in the ILN, but not the amygdala. Together, our results delineate the neural circuit underlying nocifensive behavior, providing crucial insight into the circuit mechanism underlying nociceptive information processing.