文献类型：期刊文献

中文题名：rhBMP-2对再生障碍性贫血小鼠骨髓造血恢复的促进作用

英文题名：Promoting Effects of rhBMP-2 on Aplastic Anemia Mice Bone Marrow Hematopoietic Recovery

作者：高巧艳[1];王靖[1];邢万里[1];戴凯[1];刘昌胜[1]

机构：[1]华东理工大学材料科学与工程学院,教育部医用生物材料工程研究中心,上海200237

年份：2016

卷号：42

期号：1

起止页码：42

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：纳米研究重大科学研究计划(2012CB933600)

语种：中文

中文关键词：重组人骨形态发生蛋白-2;再生障碍性贫血;造血损伤修复

外文关键词：human bone morphogenetic protein-2(rhBMP-2);aplastic anemia;hematopoietic damage repair

摘要：探究了人重组骨形态发生蛋白-2(rhBMP-2)对再障小鼠的治疗作用。采用5-FU联合白消安建立小鼠再障模型,通过干预rhBMP-2进行治疗。考察了各实验组小鼠白细胞数、骨髓单核细胞数、体重、存活率、脾系数、粒系-巨系细胞集落(CFU-GM)数以及骨髓单核细胞中CD34+细胞比例,进行股骨、脾脏HE切片分析。结果表明,相较于再障对照组,rhBMP-2治疗组小鼠的白细胞数、存活率、CFU-GM集落数及骨髓单核细胞数显著提高,并且骨髓单核细胞中的CD34+细胞含量也提高,显著改善脾脏功能和缓解骨髓抑制,证实rhBMP-2能够促进再障小鼠造血损伤的修复。
Aiming to explore the promoting effects of rhBMP-2on aplastic anemia mice bone marrow hematopoietic recovery,we built the hematopoietic injury model by 5-FU and busulfan with the interventional treatment of rhBMP-2.We analysed the number of mice peripheral white blood cells,bone marrow mononuclear cells,BM CD34+cells,weight index,survival rate,spleen coefficient,CFU-GM colony as well as the HE sections of femur and spleen tissue.The results showed that rhBMP-2can improve the index of peripheral white blood cell amount,mice survival rates,CFU-GM colony number,the bone marrow mononuclear cells number and CD34+cells ratio;rhBMP-2can also improve the function of the spleen and reduce bone marrow suppression compared with the AA control group.All results above confirmed that rhBMP-2can effectively promote the restoration of hematopoietic injury in aplastic anemia mice.